Coding

Part:BBa_K3764002:Design

Designed by: Slavil Peykov   Group: iGEM21_Bulgaria   (2021-10-21)


mRFP1 with T38stop (TAG) mutation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Tyr codon (TAC) was selected because it differs from the Amber stop codon (TAG) just by one nucleotide at 3rd position.


Source

Source - part E1010. Our version was generated by gene synthesis but it can also be produced via site-directed mutagenesis.

References